pECTO金黃色葡萄球菌染色體基因異位互補質粒 貨號：P0434
來源:
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作者:shhebio
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發布時間: 2018-04-16
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700 次瀏覽
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信息
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別名
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金黃葡萄球染體異位互補
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原核抗性
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Amp
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克隆菌株
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DH5α
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培養條件
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37℃
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質粒宿主
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金黃色葡萄球菌
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質粒用途
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蛋白表達
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原核抗性
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氨芐青霉素
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簡介
pMAD2 derivative designed for chromosomal gene ectopic complementation in Staphylococcus aureu.pECTO a Gram negative/Gram positive shuttle vector derived from pMAD2 designed to integrate DNA sequences between SAOUHSC_00278 and SAOUHSC_00279 (NCTC8325 nomenclature HG003). It carries about 1kb of both sequences. A terminator introduced between the SAOUHSC_00278 and SAOUHSC_00279 sequences prevents polar effects associated with the expression of inserted genes.This a Gram negative/Gram positive shuttle vector with two specialized replicons and antibiotic markers. pBR322 replicon and ampicillin resistance for E. coli. pE194(ts) replicon and erythromycin resistance for S. aureus. In S. aureus, the plasmid propagates at T<30°C, it becomes a suicide vector at T>37°C.
圖譜
